Evolution of nucleo-mitochondrial interactions and mitochondrial gene expression

P.I.

Golik.jpg

prof. dr hab. Paweł Golik   

pgolik(at)igib.uw.edu.pl

 

 

 

 

 

Post-docs

Dr. Karolina Łabędzka-Dmoch 

Dr. Adam Kolondra

 

Ph.D. students: 

mgr Jakub Kruszewski

mgr Jakub Piątkowski 

mgr Bartosz Zapisek

 

Research

We are interested in nuclear encoded proteins involved in RNA metabolism, particularly PPR proteins, RNA helicases and ribonucleases, in yeasts, including Saccharomyces  and Candida albicans. We also want to understand the role of RNA metabolism in the evolution of nucleo-mitochondrial interactions. We also collaborate with the mitochondrial diseases group, creating yeast models of human diseases caused by mutations in nuclear-encoded proteins involved in the mitochondrial genome expression.

 

Grants 

  • TEAM/2010-6/6; Yeasts-an evolutionary laboratory for studying nucleo-mitochondrial interactions, EU Operational Programme Innovative Economy via the Foundation for Polish Science
    team_PG_header.jpg 

  • N N303 550639; Funkcje i ewolucja białek z rodziny PPR u drożdży, Ministerstwo Nauki i Szkolnictwa Wyższego

Selected publications

  1. Lipinski KA, Puchta O, Surandranath V, Kudla M, Golik P.
    Revisiting the Yeast PPR Proteins  Application of an Iterative Hidden Markov Model Algorithm Reveals New Members of the Rapidly Evolving Family.
    Mol. Biol. Evol. 2011, 10:2935-48.
  2. Puchta O, Lubas M, Lipinski KA, Piatkowski J, Malecki M, Golik P. 
    DMR1 (CCM1/YGR150C) of Saccharomyces cerevisiae encodes an RNA-binding protein from the pentatricopeptide repeat family required for the maintenance of the mitochondrial 15S ribosomal RNA. 
    Genetics. 2010 Apr;184(4):959-73 
  3. Małecki M, Jędrzejczak R, Stępień PP, Golik P. 
    In vitro Reconstitution and Characterization of the Yeast Mitochondrial Degradosome Complex Unravels Tight Functional Interdependence. 
    J. Mol. Biol. 2007, 372:23-36.
  4. Szczepanek T, Gora M, Monteilhet C, Wysocka M, Lazowska J, Golik P. 
    In vivo analysis of the relationships between the splicing and homing activities of a group I intron-encoded I-ScaI/bi2-maturase of Saccharomyces capensis produced in the yeast cytoplasm. 
    FEMS Yeast Res. 2006 Aug;6(5):823-35. 
  5. Rogowska AT, Puchta O, Czarnecka AM, Kaniak A, Stepien PP, Golik P. 
    Balance between Transcription and RNA Degradation Is Vital for Saccharomyces cerevisiae Mitochondria: Reduced Transcription Rescues the Phenotype of Deficient RNA Degradation. 
    Mol Biol Cell. 2006 Mar;17(3):1184-93 
  6. Golik P, Zwolinska U, Stepien PP, Lazowska J. 
    The SUV3 gene from Saccharomyces douglasii is a functional equivalent of its Saccharomyces cerevisiae orthologue and is essential for respiratory growth. 
    FEMS Yeast Res. 2004 Jan;4(4-5):477-85.
  7. Golik P, Bonnefoy N, Szczepanek T, Saint-Georges Y, Lazowska J. 
    The Rieske FeS protein encoded and synthesized within mitochondria complements a deficiency in the nuclear gene. 
    Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8844-9. 

Collaborations 

  • Institute of Biochemistry and Biophysics, PAS, Poland
  • Centre de Génétique Moléculaire du CNRS, Gif-sur-Yvette, France 
  • Institut de Biochimie et Génétique Cellulaires (IBGC), Bordeaux, France