Vertebrate mitochondrial gene expression

P.I.

prof. Piotr P. Stępień

 

Ph.D. students: 

mgr Maciej Szewczyk

mgr Magdalena Wójcik, Wydział Biologii UW

 

Research

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Funding


Selected publications

  1. Rogowska AT, Puchta O, Czarnecka AM, Kaniak A, Stepien PP, Golik P. 
    Balance between Transcription and RNA Degradation Is Vital for Saccharomyces cerevisiae Mitochondria: Reduced Transcription Rescues the Phenotype of Deficient RNA Degradation. 
    Mol Biol Cell. 2006 Mar;17(3):1184-93 
  2. Minczuk M, Mroczek S, Pawlak SD, Stepien PP. 
    Human ATP-dependent RNA/DNA helicase hSuv3p interacts with the cofactor of survivin HBXIP. 
    FEBS J. 2005 Oct;272(19):5008-19. 
  3. Minczuk M, Lilpop J, Boros J, Stepien PP. 
    The 5' region of the human hSUV3 gene encoding mitochondrial DNA and RNA helicase: promoter characterization and alternative pre-mRNA splicing. 
    Biochim Biophys Acta. 2005 Jun 30;1729(2):81-7.
  4. Tomecki R, Dmochowska A, Gewartowski K, Dziembowski A, Stepien PP. 
    Identification of a novel human nuclear-encoded mitochondrial poly(A) polymerase. 
    Nucleic Acids Res. 2004 Nov 16;32(20):6001-14.
  5. Golik P, Zwolinska U, Stepien PP, Lazowska J. 
    The SUV3 gene from Saccharomyces douglasii is a functional equivalent of its Saccharomyces cerevisiae orthologue and is essential for respiratory growth. 
    FEMS Yeast Res. 2004 Jan;4(4-5):477-85.
  6. Malecki M, Stepien PP. 
    Various mechanisms of mRNA degradation 
    Postepy Biochem. 2003;49(2):58-67.
  7. Piwowarski J, Grzechnik P, Dziembowski A, Dmochowska A, Minczuk M, Stepien PP. 
    Human polynucleotide phosphorylase, hPNPase, is localized in mitochondria. 
    J Mol Biol. 2003 Jun 20;329(5):853-7.
  8. Minczuk M, Piwowarski J, Papworth MA, Awiszus K, Schalinski S, Dziembowski A, Dmochowska A, Bartnik E, Tokatlidis K, Stepien PP, Borowski P. 
    Localisation of the human hSuv3p helicase in the mitochondrial matrix and its preferential unwinding of dsDNA. 
    Nucleic Acids Res. 2002 Dec 1;30(23):5074-86.
  9. Dziembowski A, Piwowarski J, Hoser R, Minczuk M, Dmochowska A, Siep M, van der Spek H, Grivell L, Stepien PP. 
    The yeast mitochondrial degradosome. Its composition, interplay between RNA helicase and RNase activities and the role in mitochondrial RNA metabolism. 
    J Biol Chem. 2003 Jan 17;278(3):1603-11.
  10. Minczuk M, Dmochowska A, Palczewska M, Stepien PP. 
    Overexpressed yeast mitochondrial putative RNA helicase Mss116 partially restores proper mtRNA metabolism in strains lacking the Suv3 mtRNA helicase. 
    Yeast. 2002 Nov;19(15):1285-93.
  11. Dziembowski A, Stepien PP. 
    Genetic and biochemical approaches for analysis of mitochondrial degradosome from Saccharomyces cerevisiae. 
    Methods Enzymol. 2001;342:367-78.
  12. Dziembowski A, Stepien PP. 
    Analysis of 3' and 5' ends of RNA by solid-phase S1 nuclease mapping. 
    Anal Biochem. 2001 Jul 1;294(1):87-9.
  13. Dmochowska A., Kalita K., Krawczyk M., Golik P., Mroczek K., Lazowska J., Stepien P.P., Bartnik E. 
    A human putative Suv3-like RNA helicase is conserved between Rhodobacter and all eukaryotes. 
    Acta Biochim. Pol. 1999, 46(1):155-62.
  14. Wegierski T., Dmochowska A., Jablonowska A., Dziembowski A., Bartnik E., Stepien P.P. 
    Yeast nuclear PET127 gene can suppress deletions of the SUV3 or DSS1 genes: an indication of a functional interaction between 3' and 5' ends of mitochondrial mRNAs. 
    Acta Biochimica Polonica 1998, 45(4):935-40.
  15. Dmochowska A., Stankiewicz P., Golik P., Stepien P.P., Bocian E., Hansmann I., Bartnik E. 
    Assignment1 of SUPV3L1 to human chromosome band 10q22.1 by in situ hybridization. 
    Cytogen. Cell Genet. 1998, 83(1-2):84-5.
  16. Dziembowski A., Malewicz M., Minczuk M., Golik P., Dmochowska A., Stepien P.P. 
    The yeast nuclear gene DSS1, which codes for a putative RNase II, is necessary for the function of the mitochondrial degradosome in processing and turnover of RNA. 
    Mol. Gen Genet. 260(1):108-14.
  17. Dmochowska A., Golik P., Stepien P.P. 
    The novel nuclear gene Dss-1 of Saccharomyces cerevisiae is necessary for mitochondrial biogenesis. 
    Curr. Genet. 1995, 28(2):108-12.
  18. Golik P., Szczepanek T., Bartnik E., Stepien P.P., Lazowska J. 
    The S. cerevisiae nuclear SUV3 gene encoding a putative RNA helicase displays cummulative effect on the stability of intron-containing mitochondrial transcripts. 
    Curr. Genet. 1995, 28:217-24.
  19. Stepien P.P., Kokot L., Leski T., Bartnik E. 
    The suv3 nuclear gene product is required for the in vivo processing of the yeast mitochondrial 21s rRNA transcripts containing the r1 intron. 
    Curr. Genet. 1995, 27:234-8.