UPCOMING SEMINARS

Dear All,

I am pleased to invite you to the seminar at Institute of Genetics and
Biology during which Maria Bożko and Michał Świrski  will present the
results of their doctoral theses, whose supervisor is prof. Joanna
Kufel.
Results of both theses will be presented at one seminar as they are
related and concern alternative translational events revealed with
massive data reanalysis and direct experimental approaches.

Titles:
Michał Świrski - "Cryptic translational events revealed with Ribosome
Profiling and massive data reanalysis"
Maria Bożko - "Non-canonical translational events in the yeast
Saccharomyces cerevisiae"
(joint abstract below).

The seminar will be held on Friday -  December 13th at 1.30 pm, (IN
PERSON) ul. Pawińskiego 5A, at the Prof. Wacław Gajewski Auditorium
(room E), first floor (building of IBB PAN).

Please forword this information to all the person who might be
interested in. Seminars at Institute of Genetics and Biotechnology are
held in English, open to all interested persons and all are welcome to
attend.

With best regards

Agnieszka Dzikowska

JOINT ABSTRACT:
Ribosome profiling (Ribo-seq) has revolutionized the study of
translation by enabling transcriptome-wide analyses of protein
synthesis, revealing a vast landscape of translation beyond annotated
coding sequences. Despite its potential, individual studies often fall
short in uncovering rare or complex translational phenomena due to
limited sequencing depth and singular focus on specific hypotheses.
To address these limitations, we processed over 700 billion reads from
nearly all publicly available ribosome profiling datasets and their
variations, spanning more than 14,000 libraries across 120 cell lines,
43 tissues, and hundreds of experimental conditions in over 60 species.
This effort led to the development of RiboCrypt, an integrated analysis
and visualization platform that facilitates parallel analysis of
thousands of datasets, enabling the identification of cryptic
translational phenomena.
Guided by this computational framework, we experimentally investigated
novel translational mechanisms in the yeast Saccharomyces cerevisiae.
These include previously uncharacterized cases of ribosomal
frameshifting and its regulation, translation of retained introns,
alternative mitochondrial targeting signals mediated by N-terminal
extensions and upstream open reading frames, and stochastic premature
termination on sense codons.
Our findings demonstrate the power of integrating large-scale data
reanalysis with targeted experimental approaches, providing a robust
framework for exploring the complexities of translation and uncovering
alternative mechanisms of gene expression.

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